Fig. 5

Expression of nNOS in enteric neurons of the colon of transgenic mice and co-localization of nNOS and α-syn. a nNOS expression is predominant in the Auerbach’s plexus without clear age-related changes. b Expression of nNOS in distal colon at 6, 9, and 24 months. c Co-localization of nNOS and α-syn, and the nucleus (DAPI) at 24 months in Auerbach’s (AP) and Meissner’s (MP) plexuses. d Semi-quantitative analyses of nNOS in the colon of BAC mice. Two-way ANOVA (F = 2.723, p = 0.106, in ages. F = 0.031, p = 0.862). e Quantitative of co-localization between nNOS and α-syn in the colon in BAC mice. Two-way ANOVA (F = 15.262, p = 0.001, in ages. F = 1.970, p = 0.186), followed by Bonferroni adjustment post hoc tests (^*p<0.05,^***P<0.001). f Quantitative of co-localization between nNOS and α-syn at 24 months in Auerbach’s (AP) and Meissner’s (MP) plexuses. Scale bar = 150 μm in a and b; and 15 μm in c