Fig. 3

Co-localization between calretinin and α-syn in enteric neurons in the colon. a α-syn and calretinin expression in the enteric neurons increased with age and highly co-localized to each other at 6, 9, and 24 months in the proximal colon. b Expression of calretinin in the distal colon also increased with age. c Co-localization between calretinin (CR) and α-syn in the enteric nerve terminals and neuronal cell bodies in the mucosal layers (ML) and Auerbach’s (AP) and Meissner’s plexuses (MP) at 24 months. d Semi-quantitative analyses of calretinin in the colon of BAC α-syn-GFP mice. Two-way ANOVA (F = 6.277, p = 0.014, in ages. F = 0.420, p = 0.529), followed by Bonferroni adjustment post hoc tests (^*p<0.05). e Quantitative of co-localization between calretinin and α-syn in the colon in BAC α-syn-GFP mice. Two-way ANOVA (F = 1.442, p = 0.275, in ages. F = 10.840, p = 0.006). f Quantitative of co-localization between calretinin and α-syn in the mucosal layers (ML) and Auerbach’s (AP) and Meissner’s plexuses (MP) 24 months. Scale bar = 250 μm in a and b; 20 μm in c (MP, ML) and 40 μm in c (AP)