Fig. 2
The accumulation of phospho-α-syn (pS129) in enteric neurons of the colon. a. The age-dependent phosphorylation of α-syn in the Auerbach’s plexus of the proximal (Upper panels) and distal (Lower panels) colon. b. Phospho-α-syn in the colon at the age of 18 months (b1, Mucosal layer. b2, Meissner’s plexus. b3, Auerbach’s plexus). c. Partial phosphorylation (phospho-α-syn (red)) in the Auerbach’s plexus, viewed as pan α-syn expression (green) (Pearson’s correction:0.388 ± 0.100). d. The semi-quantitative analyses of phospho-α-syn intensity (immunohistochemical staining) in the Auerbach’s plexus. Two-way ANOVA (F = 14.69, p = 0.0006, in ages. F = 0.5055, p = 0.8259), followed by Bonferroni adjustment post hoc tests (**p<0.01, **p<0.001). Arrows show phospho-α-syn containing cell bodies of myenteric neurons . Scale bars = 250 μm and 100 μm (zoomed up images) in a; 500 μm and 50 μm in b; and 10 μm in c