Fig. 4

G2019S LRRK2 cells show up-regulation of RAGE protein levels. a. Western blot analysis of RAGE in doxycycline (DOX)-activated G2019S-LRRK2 expressing HEK293 cells. After 48 h transfection, cells were lysed and analyzed. b. Bar graph shows quantification of (a). Data are presented as mean ± SD for three independent experiments. ^*P < 0.05. c. Western blot analysis of RAGE in cultured primary neurons (DIV 15) from striatum of nTG mice and G2019S LRRK2 transgenic mice. d. Bar graph shows quantification of (c). Data are presented as mean ± SD for three independent experiments. ^**P < 0.01. e. Western blot analysis of RAGE in protein lysates from striatum of nTG and G2019S LRRK2 transgenic mice. Total lysates and crude membrane fraction (CMF) were analyzed. f. Bar graph shows quantification of RAGE levels from CMF lysates in (e). Data are presented as mean ± SD for three independent experiments. ^**P < 0.01. g. Bar graph shows quantification of RAGE levels from total lysates in (e). Data are presented as mean ± SD for three independent experiments. ^***P < 0.001. h. Representative images show RAGE in striatal neurons (NeuN-positive cells) from the brain sections of 3-month-old WT LRRK2 and G2019S LRRK2 transgenic mice. Neurons in the striatum region immunostained with NeuN (red) and RAGE antibodies (green). Scale bar, 20 μm i. Bar graph shows quantification of (h). Data are presented as mean ± SD for three independent experiments. ^***P < 0.001