Fig. 6
From: Identification of Ser465 as a novel PINK1 autophosphorylation site
Both disease-causing mutant PINK1 protein and Ser465A mutant PINK1 protein affects Parkin subcellular localization. a The six rows represented the subcellular localization of co-transfection of EGFP-Parkin with pKH3, pKH3-PINK1-WT, pKH3-PINK1-S465A, pKH3-PINK1-S465D, pKH3-PINK1-T313 M and pKH3-PINK1-R492X, respectively. The red fluorescence showed the subcellular localization of PINK1 protein containing HA tag in the first column; The green fluorescence illustrated subcellular localization of Parkin protein containing GFP tag in the second column; The yellow fluorescent showed the co-localization of both PINK1 (red) and Parkin (green) in the third column. b The percentage of positive cells containing colocalization of PINK1 and Parkin in six co-transfection groups of EGFP-Parkin with pKH3 vector, pKH3-PINK1-WT, pKH3-PINK1-S465A, pKH3-PINK1- S465D, pKH3-PINK1-T313 M and pKH3-PINK1-R492X. c Quantification of the percentage of PINK1/Parkin colocalization cell. (*P < 0.05, statistically significant)