Fig. 1
From: Identification of Ser465 as a novel PINK1 autophosphorylation site
Identification of PINK1 protein autophosphorylation site(s) with mass spectrometry. a Coomassie blue staining of purified GST-PINK1-WT fusion protein. The asterisk indicates the band of PINK1, 77 kDa, equivalent of the molecular weight of GST-PINK1-WT fusion protein. b Protein score histogram in Mascot with the mass spectrometry data. The horizontal axis stands for protein match score (a higher score indicating a more reliable result), and the vertical axis represents the number of matched protein. The red bar, located around 1600–1700 at the horizontal axis, suggests the autophosphorylation site. c Detailed information of the identified autophosphorylation site. d A comparison of the identified sequence with the PINK1 protein sequence (NCBI database). The modified serine site (marked by black square), referring to the autophosphorylation site, is located at the 465th serine site in PINK1. e Ser465 (in red) displays highly conserved in nine organisms