Fig. 1

SIRT1 is expressed in lumbar spinal neuronal somas, including MNs. a Representative maximal intensity projections of confocal immunohistofluorescence images of the ventral gray matter of spinal cord sections of intact rats labeled with anti-SIRT1 (red, left and right columns), anti-MAP2 (green, Neurons row), anti-GFAP (green, Astrocytes row) and anti-Iba1 (green, Microglial cells row). Merged images (right column, with DAPI staining in magenta), show that there is co-location of SIRT1 signal only in neuronal somas and nuclei, including MNs (yellow cells). Scale bar, 100 μm. b Representative maximal intensity projections of high-magnification confocal immunohistofluorescence images; labeling and color codes are the same as in panel A. In the merged images SIRT1 labeling co-localizes with MAP2 immunostaining (Neurons rows) and is also present in neuronal nuclei. SIRT1 labeling signal was not detected in GFAP (Astrocytes row) or in Iba1 (Microglial cells) immunostained cells. Scale bar, 50 μm. Images were obtained from three spinal cord slices of five animals, all of which yielded similar results