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Table 1 Comparison of NGS platforms with Sanger sequencing

From: The role of gene variants in the pathogenesis of neurodegenerative disorders as revealed by next generation sequencing studies: a review

  WGS WES Sanger sequencing
Target of sequencing Entire genome Protein coding regions (~20,000 genes) Region of interest limited to 1000 bp
Run time <7 days <7 days hours
Advantages -Comprehensive sequencing -Sequencing of all protein coding regions at reduced cost compared with WGS -High accuracy -Flexible target regions
Disadvantages -Expensive -Large amount of data, challenging to analyze -unable to detect mutations in non-coding regions -time consuming and expensive for large-scale sequencing projects