Table 1 Comparison of NGS platforms with Sanger sequencing

Target of sequencing

Entire genome

Protein coding regions (~20,000 genes)

Region of interest limited to 1000 bp

Run time

<7 days

<7 days

hours

Advantages

-Comprehensive sequencing

-Sequencing of all protein coding regions at reduced cost compared with WGS

-High accuracy -Flexible target regions

Disadvantages

-Expensive -Large amount of data, challenging to analyze

-unable to detect mutations in non-coding regions

-time consuming and expensive for large-scale sequencing projects