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Fig. 5 | Translational Neurodegeneration

Fig. 5

From: Mesencephalic astrocyte-derived neurotrophic factor reduces cell apoptosis via upregulating HSP70 in SHSY-5Y cells

Fig. 5

Effects of HSP70 blockade on the protective effects of MANF against 6-OHDA induced apoptosis in SHSY-5Y cells. a SHSY-5Y cells were pretreated with control RNAi of HSP70 for 48 h and then stimulated with 150 μM 6-OHDA or 150 μM 6-OHDA + 4 μg/mL MANF for further 48 h. Cell viability was measured by MTT. (*p < 0.05; one-way ANOVA with Bonferroni post-tests). b Representative fluorescent images for DAPI and TUNEL merged images in control group HSP70 RNAi SHSY-5Y cells; HSP70 RNAi SHSY-5Y cells treated with 150 μM 6-OHDA; HSP70 RNAi SHSY-5Y cells treated with 150 μM 6-OHDA +4 μg/mL MANF; scale bar, 100 μm. c The protective effect of MANF against 6-OHDA-induced apoptosis disappeared in HSP70 RNAi SHSH-5Y cells

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Translational Neurodegeneration

ISSN: 2047-9158

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