Skip to main content

Table 4 Kinetics of the enzyme activity in full-length KGA and its N-terminal deletion mutants

From: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration

Protein Km (mM) Vmax (μmol/min per ml)
KGA FL 21.23 ± 3.57 30.50 ± 2.18
KGA 111–669 AA 27.37 ± 2.80 34.15 ± 1.64
KGA 219–669 AA 36.61 ± 8.70 41.39 ± 5.19
KGA 311–669 AA 0.43 ± 0.28 0.82 ± 0.03
KGA 415–669 AA 1.00e-007 ± 0.21 0.65 ± 0.02