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Table 3 Kinetics of the enzyme activity in full-length GAC and its N-terminal deletion mutants

From: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration

Protein Km (mM) Vmax (μmol/min per ml)
GAC FL 27.44 ± 4.92 37.65 ± 3.18
GAC 111–598 AA 34.30 ± 3.88 41.24 ± 2.4
GAC 219–598 AA 19.77 ± 3.17 3 1.61 ± 2.14
GAC 311–598 AA 0.51 ± 0.29 0.78 ± 0.03
GAC 415–598 AA 1.00e-007 ± 0.19 0.52 ± 0.02