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Table 1 Primer sequences for amplifying full length KGA, full length GAC and all the deletions

From: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration

KGA

1AA - 669AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: GCTCGAGTTACAACAATCCATCAAG

KGA

111AA - 669AA

Forward: TGGATCCATGGAGACGGACGCGTTTGGCAAC

Reverse: GCTCGAGTTACAACAATCCATCAAG

KGA

219AA - 669AA

Forward: CGGATCCATGGTGATTCCTGACTTTATGTC

Reverse: GCTCGAGTTACAACAATCCATCAAG

KGA

311AA - 669AA

Forward: TGGATCCATGGAGCCGAGTGGACTAAGATTC

Reverse: GCTCGAGTTACAACAATCCATCAAG

KGA

415AA - 669AA

Forward: TGGATCCATGCAGCTGTGCTCCATTGAAGTG

Reverse: GCTCGAGTTACAACAATCCATCAAG

GAC

1AA - 598AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: GCTCGAGTTAGCTTTTCTCTCCCAGAC

GAC

111AA - 598AA

Forward: CCTCGAGTCCTTCAGCAATTGTATAG

Reverse: GCTCGAGTTAGCTTTTCTCTCCCAGAC

GAC

219AA - 598AA

Forward: CGGATCCATGGTGATTCCTGACTTTATGTC

Reverse: GCTCGAGTTAGCTTTTCTCTCCCAGAC

GAC

311AA - 598AA

Forward: TGGATCCATGGAGCCGAGTGGACTAAGATTC

Reverse: GCTCGAGTTAGCTTTTCTCTCCCAGAC

GAC

415AA - 598AA

Forward: TGGATCCATGCAGCTGTGCTCCATTGAAGTG

Reverse: GCTCGAGTTAGCTTTTCTCTCCCAGAC

GLS1

1AA - 550AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: GCTCGAGCCTTTGATCACCACCTTCTC

GLS1

1AA - 450AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: GCTCGAGAGGGCTCAGTACTCTTTCACCA

GLS1

1AA - 344AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: GCTCGAGTAGTGAAGTCACAACAATTGC

GLS1

1AA - 247AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: GCTCGAGTGCAACCTTTCCTCCAGACT

GLS1

1AA - 150AA

Forward: AGGATCCATGCGGCTGCGAGGCTCGGGGATGCTG

Reverse: CCTCGAGTCCTTCAGCAATTGTATAG

GLS1

219AA - 550AA

Forward: CGGATCCATGGTGATTCCTGACTTTATGTC

Reverse: GCTCGAGCCTTTGATCACCACCTTCTC