Fig. 4

Serial C-terminal deletions of both GAC and KGA proteins. a) Schematic illustration of serial C-terminal deletions of GAC and KGA was shown. b) Glutaminase activity of each of the mutant was determined by the enzyme activity assay with varying glutamine concentrations. c) C-terminal deletions of GAC and KGA were tested in saturation curve of the enzyme activity assay with a fixed glutamine concentration (50 mM). Data was plotted using non-linear regression with GraphPad Prism software. Results shown are the means ± SD of triplicate samples