Fig. 3

Serial N-terminal deletions of KGA protein. a) Schematic illustration of serial N-terminal deletions of KGA was shown. b) KGA N-terminal deletion mutant proteins were lysed and subjected to Western blotting with a specific KGA antibody for KGA detection. c) Glutaminase activity of each of the mutant was determined by the enzyme activity assay with varying glutamine concentrations. Data was plotted as mmol/L per 30 min reaction time against the glutamine concentration. Non-linear regression was performed with GraphPad Prism software. Results shown are the means ± SD of triplicate samples