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Fig. 2 | Translational Neurodegeneration

Fig. 2

From: Elevated axonal membrane permeability and its correlation with motor deficits in an animal model of multiple sclerosis

Fig. 2

Membrane permeability assessed by HRP-exclusion test. Horseradish peroxidase (HRP)-exclusion test determined amount of axonal membrane damage in healthy control mice (n = 5), pre-symptom EAE mice (n = 7), peak symptom EAE mice (n = 6), and peak symptom EAE + PEG-treated mice (n = 7). For pre-symptom EAE group, samples were taken at 8 days post-induction, before symptom emerges. The samples of peak symptom group (for both EAE and EAE + PEG-treated) were taken at 28 days post-induction. ad The images represent HRP-stained sections of spinal cord tissue from the four groups. Solid arrows denote areas in which HRP did not penetrate the cell while the open arrows point to areas depicting HRP penetration revealing increased axonal membrane permeability. e The bar graph quantifies HRP uptake in each group. The average density for the control group was 811 ± 130 axons/mm2. The peak symptom group had the highest levels of axonal damage (6147 ± 665 axons/mm2, ** P < 0.01 compared to control) while the pre-symptom group exhibited increased levels compared to the control group (3293 ± 500 axons/mm2, * P < 0.05 compared to control). In addition, HRP labeling in the EAE/PEG-treated group (1581 ± 247 axons/mm2) is significantly lower than EAE group (** p < 0.01). Scale bar = 10 mm for a, b, c and d

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