Table 1 Primers for PCR assays
From: Methylation status of DJ-1 in leukocyte DNA of Parkinson’s disease patients
Primer | Sequence (5’ > 3’) | Tm (°C) | Amplicon (bp) |
|---|---|---|---|
DJ-1- CpG1-methylation-PCR-F | GTTYGGGAGGTTTGGATTAGAGTT | 56 | 170 |
DJ-1- CpG1-methylation-PCR-R | ACRACTCRATCCCACATAATACCC | ||
DJ-1- CpG2-methylation-PCR-F | TTGYGTAGTGTGGGGTTGAGG | 58 | 141 |
DJ-1- CpG2-methylation-PCR-R | ACCRTCCAACACAAAAACACC | ||
DJ-1- CpG1-methylation-Seq-R | ACRACTCRATCCCACATAATACCC | ||
DJ-1- CpG2-methylation-Seq-R | ACCRTCCAACACAAAAACACC | ||
DJ-1- Realtime-F | CGGGGTGCAGGCTTGTAAA | 58 | 150 |
DJ-1- Realtime-R | TCCGGTTTTCCTGCTCCTTC |