Fig. 2

α-synuclein is not transmitted in anatomically connected regions. a In the top panel, sample image shows the expression pattern of hα-syn (green) and TH (red) in the sagittal sections of 1–month–old Drd1a–rtTA::tetO–SNCA bigenic mice. Topro3 was used for counter–staining (blue). In the bottom left panel, arrowheads point to Drd1–type striatal neurons that express hα-syn. The bottom right panel highlights the boxed area in the top panel. Scale bar: 1 mm. b Sample images show the staining of hα-syn (green) and TH (red) in the SNc of 1– and 18–month–old Drd1a–rtTA::tetO–SNCA bigenic mice. Arrowheads point to the hα-syn–positive puncta. Scale bar: 10 μm. c Sample image shows the staining of hα-syn (green), synaptophysin (red) and TH (blue) in the SNc of 18–month–old Drd1a–rtTA::tetO–SNCA bigenic transgenic mice. The panels at the top and right depict the distribution of different fluorophores along the Y– and X–axis. Scale bar: 10 μm