Memory and spatial learning evaluation following zinc acetate treatment. (A) Mice were trained for two days using a visual platform raised above the water level. Data represents the mean ± SEM of the time to reach the platform in seconds, for all mice in the treatment group. (B) The water level was raised and mice were trained to find the hidden platform using visual cues around the room. Each data point is the average of six trials per day per mouse in each treatment group and represented as the mean ± SEM of the time to reach the platform in seconds. Following hidden platform training, the mice were tested at (C) 2 hours and (D) 72 hours for the ability to remember where the hidden platform was located. Data is presented as the mean ± SEM of the percentage of time spent in the target quadrant for all mice in the treatment group. (E) Mice were analyzed for time spent with a novel object as a function of working memory. Data is represented as the mean ± SEM of the percentage of total time spent with the novel object for all mice in each treatment group.