Metal levels in hTau mice after six months of treatment. (A) Copper levels in the plasma were determined by a surrogate, ceruloplasmin, for both males and females and plotted as a percentage of the vehicle treated mice. ICP-MS was used to analyze levels of copper and zinc in the plasma (B and C respectively) and brain (D and E respectively). Metal levels are plotted as parts per billion (ppb) for plasma and μg of metal per gram of homogenized brain tissue. Data is represented as the mean ± SEM of all mice in the treatment group.