Copper affects tau phosphorylation. Varied copper and tetrathiomolybdate concentrations were added to the media of SH-SY5Y cells in an effort to elucidate the effects of copper on tau phosphorylation, with phosphorylation induced with copper at a concentration of 400 uM but not lower concentrations. Western blot bands for phosphorylated tau at T231 were quantified. The mean ± the SEM of the band intensity is represented. * = p <0.05, ** = p <0.01, *** = p <0.001.