Figure 2
From: RNAi-mediated knock-down of Dab and Numb attenuate Aβ levels via γ-secretase mediated APP processing
Effects of RNAi knock-down of Numb on APP processing and Aβ levels in H4-FL-APP cells. In H4-FL-APP cells, Numb siRNA treatment decreases protein levels of Numb, increases protein levels of APP-C99, and decreases Aβ levels. A. APP processing in Western blot analyses. Numb immunoblotting shows reductions in protein levels of Numb in the cells treated with Numb siRNA (columns 3 and 4) as compared to control siRNA (columns 1 and 2). FL-APP immunoblotting reveals that there is no significant difference in protein levels of FL-APP in the cells treated with control siRNA or Numb siRNA. APP-CTFs immunoblotting shows increases in protein levels of APP-C99 in the cells treated with Numb siRNA (columns 3 and 4) as compared to control siRNA (columns 1 and 2). B. Protein levels of Numb assessed by quantifying Numb in the Western blot. Numb siRNA treatment (black bar) significantly decreases protein levels of Numb as compared to control siRNA treatment (white bar) (*p < 0.05), normalized to β-actin. C. APP processing assessed by quantifying ratio of APP-C99 to FL-APP in the Western blot. Numb siRNA treatment (black bar) significantly increases ratio of APP-C99 to FL-APP as compared to control siRNA treatment (white bar) (*p < 0.05), normalized to β-actin. D. APP processing assessed by quantifying ratio of APP-C83 to FL-APP in the Western blot. Numb siRNA treatment (black bar) does not significantly increase ratio of APP-C83 to FL-APP as compared to control siRNA treatment (white bar), normalized to β-actin. E. Effects of RNAi knock-down of Numb on Aβ levels in H4-FL-APP cells. Numb siRNA treatment (black bar) decreases Aβ40 levels as compared to control siRNA treatment (white bar) (*p < 0.05).