Figure 4
From: Intracellular distribution of amyloid beta peptide and its relationship to the lysosomal system
Double immunostaining for Aβ 42 (green fluorescence) and lysosomal/late endosomal marker LAMP2 (red fluorescence) in RA differenatiated SH-SY5Y cells cultured under normal conditions or exposed to exocytosis inhibitor tetanus toxin (TeNT, 5 nM) for 24 h . Both Aβ42 and LAMP-2 specific fluorescence are increased in tetanus toxin exposed cells, and the colocalization of Aβ42 with LAMP-2 positive structures (arrow and corresponding inset) is increased after TeNT treatment. Scale bar, 5 μm.