Figure 5
Percentage of human NPC migration induced by IL-1β. Human NPCs were injected parallel to PBS, IL-1β, SDF-1 or MCP-1, 1 mm apart into the basal ganglion of SCID mice (n = 3 per group). The distance between the two injection sites was divided into five even sections as diagramed in E. A slide from each section was picked for serial confocal images for PBS (A 1-5), IL-1β (B 1-5), starting from the NPC injection site. Nestin positive cells (red) in each part were counted and the percentage of the migrating NPCs in each part was calculated as the cells in each part/ total migrated cells counted. 83.8% of NPCs in the PBS group were mainly distributed around the injection site (A 1-2, F), and no cells were found beyond the second field (A 3-5, F). NPCs migrated further towards the IL-1β injection site (B 1-5, F). NPCs migrated further to SDF-1 (C 1-5, and G) and MCP-1 (D 1-5 And H) injection sites compared with PBS injection.