Fig. 3

CPE-upregulating miRNA agomirs promote hippocampal neurogenesis and mBDNF generation in middle-aged WT mice. a, b Representative images (upper panel) of BrdU and DCX double-labelled newly generated neurons in the DG (a) or SVZ (b) of middle-aged WT mice 1 week after miRNA mimic icv injection. Scale bars, 20 µm. Quantifications of the number of BrdU⁺ and BrdU⁺DCX⁺ cells are shown in the lower panel. Data are presented as mean ± SEM, n = three mice each group. Two-tailed Student’s t-test. c, d Representative images (left) and quantification (right) of SOX2⁺CPE⁺ cells in the SGZ of middle-aged WT mice 1 week after icv injection of NC-, m10- or m37-miRNA mimics (c) or agomirs (d). The inset in (c) shows magnified image of SOX2⁺CPE⁺ cells in the SGZ. Ho, Hoechst. Scale bars, 50 µm. Data are presented as mean ± SEM, n = three mice each group. e Representative images of BrdU and DCX double-labelled newly generated neurons in the DG of the brain from middle-aged WT mice 1 week after miRNA agomir icv injection. Scale bars, 20 µm. The white boxes represent the area imaged with a higher magnification. Quantification of BrdU⁺ and BrdU⁺DCX⁺ cells in the DG is shown on the right. Data are presented as mean ± SEM, n = three mice each group. f Representative images of mBDNF expression in the DG of middle-aged WT mice 1 week after miRNA agomir icv injection. Ho, Hoechst. Scale bar, 20 µm. Normalized mBDNF fluorescence intensity in the DG is shown on the right. Data are presented as mean ± SEM, n = three mice each group. For (b–f), data were analyzed with one-way ANOVA