Fig. 1

Analysis of distinct α-syn species derived from homogenates or sarkosyl-pelleting fractions of three distinct brain regions (cingulate gyrus, putamen, amygdala) from n = 3 independent control, PD and MSA subjects, respectively. a SDS-PAGE analysis of total α-syn (MJFR1) depicts a monomeric band while amyloids are retained in the wells. b Filter-blot analysis of total (MJFR1) α-syn retained on the top nitrocellulose and the underneath PVDF membranes in the supernatant (S) and pellet (P) sarkosyl-pelleting fractions. c Drop-blot analysis of total (MJFR1, green) and non-amyloid (Syn1, red) α-syn in the same fractions. Samples were fixed with 4% PFA after dropping. d Left: same drop-blot analysis on total brain homogenates before pelleting. Middle: samples are plotted with their respective Syn1 (x-axis) and MJFR1 (y-axis) signal intensities. Right: proportion of aggregated α-syn calculated as indicated (mean ± SD). e α-Syn amounts measured with our in-house ELISA (MJFR1 capture, MJFR14 detection) in total brain homogenates (left) and sarkosyl-insoluble pellets (right) as µg of α-syn per mg of protein in total brain homogenates (mean ± SD). Significant differences obtained from Tukey-corrected two-way ANOVAs are represented (*P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001)