Fig. 4

Proteomic profiling analysis. a Heat mapping of WTC11, H9, CS07, CS53, and dF-CM groups. The dF-CM is farthest from the other groups. b The Venn diagram of the proteins that were present at different levels in studied conditioned media. c The KEGG pathway list according to biological functions. d Heat mapping of representative KEGG pathways, iP3K-AKT signaling pathway (P = 5.00E−04) and TGF-β signaling pathway (P = 9.30E−03). e Heat mapping of negative regulators of apoptotic process (P = 3.00E−06) and inflammatory response (P = 2.60E−06). *Negative regulation of neuronal apoptosis (P = 8.00E−03). f Heat mapping of inflammation-related proteins. The levels of these proteins were lower in PSC-CM than in dF-CM. g Heat mapping of Heparin-bound proteins and neuroprotective proteins. h Volcano plot of the PSC-CM and dF-CM. The red dots represent differently expressed proteins (P < 0.05; FC > 1.75), while the grey dots represent proteins with P > 0.05