Fig. 12
Fluoro-Jade C (FJC) staining in the SN pars compacta (a) and CA1 of hippocampus (b) of rats with 7-day chronic intraperitoneal injection of Control (DMSO/saline), CPA, DPCPX, 1-aminoindan, 2-aminoindan, DPCPX + CPA, 1-aminoindan + CPA, and 2-aminoindan + CPA. Scale bar 50 μm. Summary bar graphs show significant increases in the relative fluorescence intensity of FJC staining in pars compacta after CPA, 2-aminoindan, and CPA + 2-aminonindan treatments (a). In contrast, only CPA and CPA + 2-aminoindan treatments significantly increased FJC fluorescence in the CA1 hippocampal neurons (b). FJC fluorescence intensity in a 100 × 100 μm2 region was normalized to the control group (100%). Values are shown as mean ± SEM. The average FJC fluorescence values were obtained from n = 4 independent experiments. ns, non-significant; *P < 0.05; **P < 0.01; and ***P < 0.001 (one-way ANOVA followed by Student–Newman–Keuls post-hoc multiple comparison test)