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Table 5 Kinetics of the enzyme activity in full-length GAC, KGA, and their C-terminal deletion mutants

From: Serial deletion reveals structural basis and stability for the core enzyme activity of human glutaminase 1 isoforms: relevance to excitotoxic neurodegeneration

Protein

Km (mM)

Vmax (μmol/min per ml)

GAC FL

28.62 ± 5.37

45.37 ± 4.08

KGA FL

21.69 ± 3.55

35.44 ± 2.48

GLS1 1–150 AA

0.63 ± 0.32

1.30 ± 0.05

GLS1 1 - 247AA

0.69 ± .039

0.92 ± 0.05

GLS1 1–344 AA

0.44 ± 0.26

0.84 ± 0.04

GLS1 1–450 AA

0.66 ± 0.39

0.76 ± 0.03

GLS1 1–550 AA

28.13 ± 3.30

46.14 ± 2.60

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Translational Neurodegeneration

ISSN: 2047-9158

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