Curcumin inhibition of JNKs prevents dopaminergic neuronal loss in a mouse model of Parkinson’s disease through suppressing mitochondria dysfunction
© Pan et al.; licensee BioMed Central Ltd. 2012
Received: 29 May 2012
Accepted: 13 August 2012
Published: 20 August 2012
Curcumin,a natural polyphenol obtained from turmeric,has been implicated to be neuroprotective in a variety of neurodegenerative disorders although the mechanism remains poorly understood. The results of our recent experiments indicated that curcumin could protect dopaminergic neurons from apoptosis in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson’s disease (PD). The death of dopaminergic neurons and the loss of dopaminergic axon in the striatum were significantly suppressed by curcumin in MPTP mouse model. Further studies showed that curcumin inhibited JNKs hyperphosphorylation induced by MPTP treatment. JNKs phosphorylation can cause translocation of Bax to mitochondria and the release of cytochrome c which both ultimately contribute to mitochondria-mediated apoptosis. These pro-apoptosis effect can be diminished by curcumin. Our experiments demonstrated that curcumin can prevent nigrostriatal degeneration by inhibiting the dysfunction of mitochondrial through suppressing hyperphosphorylation of JNKs induced by MPTP. Our results suggested that JNKs/mitochondria pathway may be a novel target in the treatment of PD patients.
Parkinson’s disease (PD) is second only to Alzheimer’s disease (AD) as the most common and debilitating age-associated human neurodegenerative disorder. A host of environmental, genetic, and immune cues have been associated with the onset of this disease . Clinical symptoms of PD include tremor, bradykinesia, rigidity, and postural instability [2, 3]. Pathologically, it is characterized by gliosis and progressive degeneration of the dopaminergic neurons associated with the presence of intracytoplasmic inclusions (Lewy bodies) in the substantia nigra pars compacta (SNc) [2, 3]. The symptoms of PD can be alleviated by drugs that enhance dopamine function, among which L-dopa is considered the most effective one. However, L-dopa fails to halt the progression of PD. Aside from having undesirable side effects, such as motor fluctuations and dyskinesias, the therapeutic effect of L-dopa diminishes after about two years of treatment . Moreover, long term use of L-dopa may actually damage neurons, accelerating neuronal apoptosis. Since programmed cell death plays a key role in the neurodegenerative processes in PD , new generation of neuroprotective agents against apoptosis may improve the prognosis of PD.
Curcumin has been implicated to be neuroprotective in a variety of neurodegenerative disorders such as AD and cerebral ischemi [6, 7]. Epidemiological evidence from India has related the huge consumption of turmeric (curcumin is its essential component) to its lowest prevalence rates of AD and PD in the world . As a matter of fact, curcumin is now in Phase II clinical trials for AD . Curcumin has been reported to be a good inhibitor of c-Jun N-terminal kinase (JNK) mediated gene transcription . JNK is a important member of mitogen-activated protein kinases (MAPK) family, which can be activated by a variety of stimuli including neurotoxic insults, environmental stress and apoptotic agents [11–13]. JNK is composed of three different isoforms, JNK1, JNK2 and JNK3. In contrast to JNK1 and JNK2, which are ubiquitously expressed, JNK3 is largely restricted to the brain and is most consistently associated with neuronal death  Our previous studies and others suggested that JNK plays an important role in mediating MPTP-induced neurotoxicity. CEP1347, a specific JNK pathway inhibitor, attenuates the loss of nigrostriatal dopaminergic neurons after the exposure to MPTP  SP600125 (a selective inhibitor of JNK) prevents dopaminergic neurons from death and decreases the loss of catecholamines in the striatum  by partially inhibiting JNK pathway. Therefore, it is reasonable to assume that blockade of JNK pathway may prevent or effectively slow down the progression of PD. Nevertheless, an understanding of the molecular mechanisms by which JNK regulates apoptosis should provide insights into the treatment of PD.
Previous studies demonstrated that JNK can promote cell death by regulating the activation of substrates, such as Bcl-2 family members . The Bcl-2/Bax heterodimer is the active component for death protection [18, 19]. Phosphorylation of Bcl-2 may possibly release Bax from Bcl-2/Bax dimmers [20–22]. The preapototic protein Bax forms pores in the outer mitochondrial membrane to release cytochrome c , thus promoting apoptosis,. On death induction, cytochrome c not only translocates into the cytosol, but furthermore can be abundantly detected in the extracellular medium. Thus, release of cytochrome c is considered as an indication of mitochondrial dysfunction . It is therefore possible that through regulating the activation of some Bcl-2 family members, activated JNK pathway increase mitochondrial membrane permeability and the subsequent release of apoptogenic factors, which could ultimately contribute to mitochondria mediated apoptosis.
Whether curcumin could inhibit the abnormal activation of JNK induced by MPTP, thus prevent the triggering of a series downstream effects that lead to apoptosis is unknown. In this study, the inhibitory effect of curcumin to the MPTP-induced activation of JNK was evaluated. Using a MPTP-induced PD mice model, we demonstrated that curcumin could suppress the activation of the JNK in PD mice induced by MPTP. Furthermore we demonstrated that curcumin could decrease MPTP-induced injuries to dopaminergic cell bodies and terminals via inhibiting mitochondria mediated apoptosis.
Materials and methods
Studies were conducted in male C57BL/6 mice (8 ~ 10 weeks old, weighing 19 ~ 22 g). Five animals were housed per cage in a temperature-controlled (25°C) room under a 12:12-h light:dark cycle with ad libitum access to food and water for 1 week before the experiment. The mice were injected intraperitoneally (i.p.) 5 times (for five consecutive days) with 30 mg/kg/day MPTP-HCl (Sigma, St. Louis, MO) or a corresponding volume of saline alone. One day after the last MPTP injection, the curcumin treated mice groups were treated with an intraperitoneal injection of curcumin (sigma) at 50 mg/kg/day for five consecutive days. Equal dose of DMSO instead of curcumin were given to the control group. At the indicated time points, the animals were killed, and their brains were processed for further analysis.
Mice were perfusion-fixed on Day 10 with 4% paraformaldehyde in 0.1 M sodium phosphate buffer (pH 7.4). Brains were removed quickly and further fixed with the same fixation solution at 4°C overnight. Post-fixed brains were embedded by paraffin, followed by preparation of coronal sections using a microtome. The paraffin-embedded brain sections were deparaffinized with xylene and rehydrated by ethanol at graded concentrations of 100-70% (v/v), followed by washing with water.
Immunoreactivity was determined by the avidin–biotin–peroxidase method. Briefly, sections were deparaffinized with xylene and rehydrated by ethanol at graded concentrations and distilled water. High-temperature antigen retrieval was performed in 1 mM citrate buffer for 15 min. To block endogenous peroxidase activity, sections were incubated for 30 min in 1% H2O2. After being blocked with 5%(v/v) normal goat serum in PBS for 1 h at 37°C, sections were incubated with a mouse polyclonal antibody against tyrosine hydroxylase (TH, 1:8000) at 4°C for 24 hours. These sections were then incubated with biotinylated goat-anti-mouse secondary antibody overnight and subsequently with avidin–conjugated horseradish peroxidase for 1 h at 37°C. Finally, sections were incubated with peroxidase substrate diaminobenzidine (DAB) until desired stain intensity developed .
Mitochondria isolated from ischemic and reperfused myocardial tissue in several kinds of buffer by density centrifugation was resuspended and fixed with 2% glutaraldehyde in 0.1 M PBS buffer. Mitochondria were post-fixed utilizing 1% OsO4. En bloc staining with uranyl acctate was followed by dehydration and embedding. Embedded samples were sectioned and affixed to grids according to standard protocols. Mitochondrial ultrastructure was then evaluated by transmission microscopy.
Immunoprecipitation and immunoblotting
Tissue homogenates (400 μg of protein) were diluted four-fold with 50 mM HEPES buffer (pH 7.4), containing 10% glycerol, 150 mM NaCl, 1% Triton X-100, 0.5% NP-40, and 1 mM each of EDTA, EGTA, PMSF and Na3VO4. Samples were preincubated for 1 h with 20 μl protein A sepharose CL-4B (Amersham, Uppsala, Sweden) at 4°C, and then centrifuged to remove proteins adhered non-specifically to protein A. The supernatants were incubated with 1-2 μg primary antibodies for 4 h or overnight at 4°C. Protein A was added to the tube for another 2 h incubation. Samples were centrifuged at 10,000 × g for 2 min at 4°C and the pellets were washed with HEPES buffer for three times. Bound proteins were eluted by boiling at 100°C for 5 min in SDS-PAGE loading buffer and then isolated by centrifugation. The supernatants were used for immunoblotting analysis. Proteins were separated on polyacrylamide gels and then electrotransferred onto a nitrocellulose membrane (Amersham, Buckinghamshire, UK). After being blocked for 3 h in Tris-buffered saline with 0.1% Tween-20 (TBST) and 3% bovine serum albumin (BSA), membranes were incubated overnight at 4°C with primary antibodies in TBST containing 3% BSA. After washing for 30 min in TBS with gentle agitation, the membrane was incubated with horseradish peroxidase-conjugated anti-mouse/rabbit IgG secondary antibody at room temperature for 2 h ([26–28]). Signals were developed using ECL Western Blotting Detection kit (Amersham-Pharmacia Biotech, Little Chalfont, UK). Band intensities were quantified by densitometric analyses using an AxioCam digital camera (ZEISS, CTED PROOF Germany) and a KS400 photo analysis system (Version 3.0).
Values were expressed as mean S.D. and obtained from at least six independent experiemnts. Statistical analysis of the results was carried out by Student’s t-test or one-way analysis of the variance (ANOVA) followed by the Duncan’s new multiple range method or Newman-Keuls test. P-values less than 0.05 were considered significant.
Effects of curcumin on MPTP-induced loss of dopaminergic neurons
To investigate whether treatment of curcumin would have neuroprotection against MPTP-induced dopaminergic neuronal death, C57BL/6 mice were subjected MPTP lesion. Mice were treated with curcumin or DMSO by injection for 5 consecutive days after last MPTP injection.
Electron micrographs analysis of morphology of mitochondria isolated
Effects of curcumin on MPTP-treated activation and expression of JNKs
JNK signaling is an important contributor to MPTP-induced apoptosis, but JNK and its isoforms (JNK1, JNK2, and JNK3) have distinct roles. As aforementioned, targeted disruption of the Jnk3 gene not only reduced the downstream effector c-Jun phosphorylation, but also remarkably protected mice from brain injury after MPTP lesion. These studies indicate the functional diversity of JNK isoforms and suggest that JNK3 is a critical component of MPTP-induced JNK signaling and neuronal apoptosis.
Curcumin inhibits the phosphorylation of Bcl-2 and the release of Bax from Bcl-2/Bax dimmers
Curcumin attenuates Bax translocation and the release of cytochrome c
The most direct evidence for disrupted mitochondrial metabolism has come from studies of autopsy tissue and other tissue samples and in vitro cell cultures derived from patients with PD . Mitochondrial dysfunction, due to either environmental or genetic factors, can trigger the apoptotic death of dopaminergic cells in PD. As a matter of fact, results from western blot and electron microscope indicated that cytochrome c releasing and mitochondrial intact were destroyed in MPTP mice. The Bcl-2/Bax heterodimer is the active component for death protection. Previous studies have also indicated that phosphorylation inactivates Bcl-2, thus promoting apoptosis, possibly by freeing Bax from Bcl-2/Bax dimmers [20–22] ,The proapoptotic protein Bax released from Bcl-2/Bax dimmers and act as the channels for either ions or proteins by forming pores in the outer mitochondrial membrane that release cytochrome c . The phosphorylation of Bcl-2 is regulated by JNK [32, 33].
A large and growing of evidence suggests that the JNK pathway can function in a pro-apoptotic manner. In vivo, the small molecule CEP-1347 inhibits MPTP-mediated JNK signaling at a dose that attenuates MPTP-mediated nigrostriatal dopaminergic loss . In addition, SP600125 also increases striatal catecholamine concentrations, resulting in behavioral changes . As a natual inhibitor of JNK, curcumin, the essential extract from turmeric, is well esablished to exert neuroprotection in animal models of degenerative diseases such as cerebral ischemia, Alzheimer’s disease [9, 34, 35]. Our previous study and others suggested that JNK3 plays a more important role than JNK1/2 in neuron death and may serve as a potential target for neuroprotective therapies. Ablation of JNKs can not only protect dopaminergic neurons against MPTP-induced neurodegeneration but also improve the motor function in mouse model of PD . As a matter of fact, results from western blot and immunoprecipitation show high fidelity to our hypothesis that curcumin could inhibiteJNKs activation.
Our study also shows that curcumin could attenuate the phosphorylation of Bcl-2 proteins, increase the interaction of Bcl-2 with Bax, and prevent Bax from translocating to sytosol induced by MPTP. Furthermore, curcumin keeps the mitochondria integrity and preserves cytochrome c loss from mitochondria which normally is considered critical role in apoptosis MPTP-intoxicated mice. Since curcumin treatment can inhibit the activation of JNKs mitochondria pathway induced by MPTP lesion, we inferred that application of curcumin would also restore dopaminergic terminals as well as SNc cell bodies from degeneration. Our present study indicated that curcumin actually had the ability to prevent dopaminergic neurons from degeneration following MPTP insult. At the same time, results from TUNEL provided strong evidence that curcumin could protect the dopaminergic neurons from apoptosis. Moreover, curcumin is the major monomer from turmeric extract. Turmeric has been utilized in traditional Indian cuisine and medicine without any known major toxic effect . Thus, the protective effect of curcumin shown here is likely clinically relevant, as significant neuroprotection was achieved when curcumin was administered after the onset of PD. At the same time, we can conclude that selective inhibition of JNKs mitochondria pathway activation may be of therapeutic benefit for PD patients.
Substantia nigra pars compacta
Mitogen-activated protein kinase
c-Jun N-terminal protein kinase
Bovine serum albumin
3-(N-morpholino) propanesulfonic acid
Tris-buffered saline with 0.1% Tween 20
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis.
This work was supported by grants from the National Program of Basic Research (2010CB945200, 2011CB504104) of China, National Natural Science Fund (34900454, 30900454, 30971031), Key Discipline Program of Shanghai Municipality (S30202), Shanghai Key Project of Basic Science Research (10411954500), and Program for Outstanding Medical Academic Leader (LJ 06003). Research Fund for the Doctoral Program of Higher Education of China (20090073120090) Special funding for original sci-tech research supported by Shanghai Municipal Education Commission ( 09YZ87).
- Caslake R, Moore JN, Gordon JC, Harris CE, Counsell C: Changes in diagnosis with follow-up in an incident cohort of patients with parkinsonism. J Neurol Neurosurg Psychiatry 2008, 79: 1202-1207. 10.1136/jnnp.2008.144501View ArticlePubMed
- Gaig C, Iranzo A, Tolosa E, Vilaseca I, Rey MJ, Santamaria J: Pathological description of a non-motor variant of multiple system atrophy. J Neurol Neurosurg Psychiatry 2008, 79: 1399-1400. 10.1136/jnnp.2008.145276View ArticlePubMed
- Jankovic J: Parkinson's disease: clinical features and diagnosis. J Neurol Neurosurg Psychiatry 2008, 79: 368-376. 10.1136/jnnp.2007.131045View ArticlePubMed
- Zhou L, Welsh AM, Chen D, Koliatsos VE: NMDA inhibitors cause apoptosis of pyramidal neurons in mature piriform cortex: evidence for a nitric oxide-mediated effect involving inhibitory interneurons. Neuropharmacology 2007, 52: 1528-1537. 10.1016/j.neuropharm.2007.02.013PubMed CentralView ArticlePubMed
- Ziv I, Zilkha-Falb R, Offen D, Shirvan A, Barzilai A, Melamed E: Levodopa induces apoptosis in cultured neuronal cells–a possible accelerator of nigrostriatal degeneration in Parkinson's disease? Mov Disord 1997, 12: 17-23. 10.1002/mds.870120105View ArticlePubMed
- Wang Q, Sun AY, Simonyi A, Jensen MD, Shelat PB, Rottinghaus GE, MacDonald RS, Miller DK, Lubahn DE, Weisman GA, Sun GY: Neuroprotective mechanisms of curcumin against cerebral ischemia-induced neuronal apoptosis and behavioral deficits. J Neurosci Res 2005, 82: 138-148. 10.1002/jnr.20610View ArticlePubMed
- Yang F, Lim GP, Begum AN, Ubeda OJ, Simmons MR, Ambegaokar SS, Chen PP, Kayed R, Glabe CG, Frautschy SA, Cole GM: Curcumin inhibits formation of amyloid beta oligomers and fibrils, binds plaques, and reduces amyloid in vivo. J Biol Chem 2005, 280: 5892-5901.View ArticlePubMed
- Giri RK, Rajagopal V, Kalra VK: Curcumin, the active constituent of turmeric, inhibits amyloid peptide-induced cytochemokine gene expression and CCR5-mediated chemotaxis of THP-1 monocytes by modulating early growth response-1 transcription factor. J Neurochem 2004, 91: 1199-1210. 10.1111/j.1471-4159.2004.02800.xView ArticlePubMed
- Dhillon N, Aggarwal BB, Newman RA, Wolff RA, Kunnumakkara AB, Abbruzzese JL, Ng CS, Badmaev V, Kurzrock R: Phase II trial of curcumin in patients with advanced pancreatic cancer. Clin Cancer Res 2008, 14: 4491-4499. 10.1158/1078-0432.CCR-08-0024View ArticlePubMed
- Collett GP, Campbell FC: Curcumin induces c-jun N-terminal kinase-dependent apoptosis in HCT116 human colon cancer cells. Carcinogenesis 2004, 25: 2183-2189. 10.1093/carcin/bgh233View ArticlePubMed
- Becker EB, Bonni A: Pin1 mediates neural-specific activation of the mitochondrial apoptotic machinery. Neuron 2006, 49: 655-662. 10.1016/j.neuron.2006.01.034View ArticlePubMed
- Huang C, Rajfur Z, Borchers C, Schaller MD, Jacobson K: JNK phosphorylates paxillin and regulates cell migration. Nature 2003, 424: 219-223. 10.1038/nature01745View ArticlePubMed
- Yujiri T, Sather S, Fanger GR, Johnson GL: Role of MEKK1 in cell survival and activation of JNK and ERK pathways defined by targeted gene disruption. Science 1998, 282: 1911-1914.View ArticlePubMed
- Kuan CY, Whitmarsh AJ, Yang DD, Liao G, Schloemer AJ, Dong C, Bao J, Banasiak KJ, Haddad GG, Flavell RA, Davis RJ, Rakic P: A critical role of neural-specific JNK3 for ischemic apoptosis. Proc Natl Acad Sci U S A 2003, 100: 15184-15189. 10.1073/pnas.2336254100PubMed CentralView ArticlePubMed
- Saporito MS, Brown EM, Miller MS, Carswell S: CEP-1347/KT-7515, an inhibitor of c-jun N-terminal kinase activation, attenuates the 1-methyl-4-phenyl tetrahydropyridine-mediated loss of nigrostriatal dopaminergic neurons In vivo. J Pharmacol Exp Ther 1999, 288: 421-427.PubMed
- Wang W, Shi L, Xie Y, Ma C, Li W, Su X, Huang S, Chen R, Zhu Z, Mao Z, Han Y, Li M: SP600125, a new JNK inhibitor, protects dopaminergic neurons in the MPTP model of Parkinson's disease. Neurosci Res 2004, 48: 195-202. 10.1016/j.neures.2003.10.012View ArticlePubMed
- Lam D, Dickens D, Reid EB, Loh SH, Moisoi N, Martins LM: MAP4K3 modulates cell death via the post-transcriptional regulation of BH3-only proteins. Proc Natl Acad Sci U S A 2009, 106: 11978-11983. 10.1073/pnas.0900608106PubMed CentralView ArticlePubMed
- Rezende AC, Vieira AS, Rogerio F, Rezende LF, Boschero AC, Negro A, Langone F: Effects of systemic administration of ciliary neurotrophic factor on Bax and Bcl-2 proteins in the lumbar spinal cord of neonatal rats after sciatic nerve transection. Braz J Med Biol Res 2008, 41: 1024-1028. LID - S0100-879X2008005000052 [pii] 10.1590/S0100-879X2008005000052View ArticlePubMed
- Zhang Z, Lapolla SM, Annis MG, Truscott M, Roberts GJ, Miao Y, Shao Y, Tan C, Peng J, Johnson AE, Zhang XC, Andrews DW, Lin J: Bcl-2 homodimerization involves two distinct binding surfaces, a topographic arrangement that provides an effective mechanism for Bcl-2 to capture activated Bax. J Biol Chem 2004, 279: 43920-43928. 10.1074/jbc.M406412200PubMed CentralView ArticlePubMed
- Biswas SC, Shi Y, Sproul A, Greene LA: Pro-apoptotic Bim induction in response to nerve growth factor deprivation requires simultaneous activation of three different death signaling pathways. J Biol Chem 2007, 282: 29368-29374. 10.1074/jbc.M702634200View ArticlePubMed
- Liu XM, Pei DS, Guan QH, Sun YF, Wang XT, Zhang QX, Zhang GY: Neuroprotection of Tat-GluR6-9c against neuronal death induced by kainate in rat hippocampus via nuclear and non-nuclear pathways. J Biol Chem 2006, 281: 17432-17445. 10.1074/jbc.M513490200View ArticlePubMed
- Pei DS, Wang XT, Liu Y, Sun YF, Guan QH, Wang W, Yan JZ, Zong YY, Xu TL, Zhang GY: Neuroprotection against ischaemic brain injury by a GluR6-9c peptide containing the TAT protein transduction sequence. Brain 2006, 129: 465-479.View ArticlePubMed
- Mohan J, Gandhi AA, Bhavya BC, Rashmi R, Karunagaran D, Indu R, Santhoshkumar TR: Caspase-2 triggers Bax-Bak-dependent and -independent cell death in colon cancer cells treated with resveratrol. J Biol Chem 2006, 281: 17599-17611. 10.1074/jbc.M602641200View ArticlePubMed
- Borutaite V, Jekabsone A, Morkuniene R, Brown GC: Inhibition of mitochondrial permeability transition prevents mitochondrial dysfunction, cytochrome c release and apoptosis induced by heart ischemia. J Mol Cell Cardiol 2003, 35: 357-366. 10.1016/S0022-2828(03)00005-1View ArticlePubMed
- Pan J, Wang G, Yang HQ, Hong Z, Xiao Q, Ren RJ, Zhou HY, Bai L, Chen SD: K252a prevents nigral dopaminergic cell death induced by 6-hydroxydopamine through inhibition of both mixed-lineage kinase 3/c-Jun NH2-terminal kinase 3 (JNK3) and apoptosis-inducing kinase 1/JNK3 signaling pathways. Mol Pharmacol 2007, 72: 1607-1618. 10.1124/mol.107.038463View ArticlePubMed
- Pan J, Zhang QG, Zhang GY: The neuroprotective effects of K252a through inhibiting MLK3/MKK7/JNK3 signaling pathway on ischemic brain injury in rat hippocampal CA1 region. Neuroscience 2005, 131: 147-159. 10.1016/j.neuroscience.2004.09.031View ArticlePubMed
- Pan J, Pei DS, Yin XH, Hui L, Zhang GY: Involvement of oxidative stress in the rapid Akt1 regulating a JNK scaffold during ischemia in rat hippocampus. Neurosci Lett 2006, 392: 47-51. 10.1016/j.neulet.2005.08.057View ArticlePubMed
- Pan J, Zhao YX, Wang ZQ, Jin L, Sun ZK, Chen SD: Expression of FasL and its interaction with Fas are mediated by c-Jun N-terminal kinase (JNK) pathway in 6-OHDA-induced rat model of Parkinson disease. Neurosci Lett 2007, 428: 82-87. 10.1016/j.neulet.2007.09.032View ArticlePubMed
- Eckert A, Keil U, Marques CA, Bonert A, Frey C, Schussel K, Muller WE: Mitochondrial dysfunction, apoptotic cell death, and Alzheimer's disease. Biochem Pharmacol 2003, 66: 1627-1634. 10.1016/S0006-2952(03)00534-3View ArticlePubMed
- Henchcliffe C, Beal MF: Mitochondrial biology and oxidative stress in Parkinson disease pathogenesis. Nat Clin Pract Neurol 2008, 4: 600-609.View ArticlePubMed
- Szabo I, Bock J, Grassme H, Soddemann M, Wilker B, Lang F, Zoratti M, Gulbins E: Mitochondrial potassium channel Kv1.3 mediates Bax-induced apoptosis in lymphocytes. Proc Natl Acad Sci U S A 2008, 105: 14861-14866. 10.1073/pnas.0804236105PubMed CentralView ArticlePubMed
- Attalla H, Westberg JA, Andersson LC, Adlercreutz H, Makela TP: 2-Methoxyestradiol-induced phosphorylation of Bcl-2: uncoupling from JNK/SAPK activation. Biochem Biophys Res Commun 1998, 247: 616-619. 10.1006/bbrc.1998.8870View ArticlePubMed
- Srivastava RK, Mi QS, Hardwick JM, Longo DL: Deletion of the loop region of Bcl-2 completely blocks paclitaxel-induced apoptosis. Proc Natl Acad Sci U S A 1999, 96: 3775-3780. 10.1073/pnas.96.7.3775PubMed CentralView ArticlePubMed
- Narang H, Krishna M: Inhibition of radiation induced nitration by curcumin and nicotinamide in mouse macrophages. Mol Cell Biochem 2005, 276: 7-13. 10.1007/s11010-005-2241-yView ArticlePubMed
- Wang SL, Li Y, Wen Y, Chen YF, Na LX, Li ST, Sun CH: Curcumin, a potential inhibitor of up-regulation of TNF-alpha and IL-6 induced by palmitate in 3 T3-L1 adipocytes through NF-kappaB and JNK pathway. Biomed Environ Sci 2009, 22: 32-39. 10.1016/S0895-3988(09)60019-2View ArticlePubMed
- Hunot S, Vila M, Teismann P, Davis RJ, Hirsch EC, Przedborski S, Rakic P, Flavell RA: JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease. Proc Natl Acad Sci U S A 2004, 101: 665-670. 10.1073/pnas.0307453101PubMed CentralView ArticlePubMed
- Jagatha B, Mythri RB, Vali S, Bharath MM: Curcumin treatment alleviates the effects of glutathione depletion in vitro and in vivo: therapeutic implications for Parkinson's disease explained via in silico studies. Free Radic Biol Med 2008, 44: 907-917. 10.1016/j.freeradbiomed.2007.11.011View ArticlePubMed
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