Fig. 2

CPE promotes adult neurogenesis and BDNF generation in the hippocampus in vivo. a Representative images and quantification of SOX2⁺CPE⁺ cells in the DG of middle-aged WT mice 1 week after LV-shNC, shCPE_1 or shCPE_2 injection. Scale bar, 10 µm. Data are presented as mean ± SEM, n = three mice each group. One-way ANOVA. b Representative images and quantification of BrdU and DCX double-labelled newly generated neurons in the DG of the same brain from middle-aged WT mice 1 week after LV-shNC (left DG), shCPE_1 or shCPE_2 (right DG) injection. Scale bar, 20 µm. Data are presented as mean ± SEM, n = three mice each group. One-way ANOVA. c Representative images and normalized fluorescence intensity of mBDNF expression in the DG of middle-aged WT mice 1 week after LV-shNC (left DG) or shCPE_2 (right DG) injection. Scale bar, 10 µm. Data are presented as mean ± SEM, n = three mice each group. d Western blotting analyses and relative quantification of proteins extracted from the mixed tissues of hippocampus from three middle-aged WT mice 1 week after LV-shNC or shCPE_2 injection. Data are presented as mean ± SEM from three mice each group. e Representative images and quantification of BrdU and DCX double-labelled newly generated neurons in the DG of the same brain from middle-aged WT mice 1 week after LV-vector (left DG) or LV-CPE (right DG) injection. Scale bar, 20 µm. Data are presented as mean ± SEM, n = six mice each group. f Representative images and normalized fluorescence intensity of mBDNF expression in the DG of middle-aged WT mice 1 week after LV-vector (left DG) or LV-CPE (right DG) injection. Scale bar, 20 µm. Data are presented as mean ± SEM, n = three mice each group. g Western blotting analyses and relative quantification of proteins extracted from cultured primary NSCs isolated from hippocampus and infected with lentivirus vector or lentivirus expressing CPE. Data are presented as mean ± SEM from three independent experiments. For (c–g), data were analyzed with two-tailed Student’s t-test